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2004 Bone Fluid Flow Workshop
2004 Bone Fluid Flow Workshop attendees
TOP ROW (left to right): Ed Guo, Dajun Zhang, Subrata Saha, Shelly Weinbaum, Howard Winet, Melissa Knothe Tate, Chris Jacobs, Larry Katz,
Dave Fyhrie, Michael Liebschner, Robert Guldberg, Stephen Harris, Aviral Vatsa, Heidi Ploeg, Shijing Qiu; MIDDLE ROW: Anita Saldanah, Sylvana Garcia, Jenneke Klein-Nulend, Liyun Wang,
Steve Cowin, Lidan You, Laoise McNamara, Sirishi Burra, Priscilla Cherian, Roland Steck, Lynda Bonewald, Susannah Fritton; BOTTOM ROW: Yilin Wang, Cesare Ciani, Yi Xia, Suzanne Ferreri,
Hoyan Lam, Yuefen Han, Ron Zernicke, Yi-Xian Qin.
Overview
The Sixth Bone Fluid Flow Workshop, organized by Dr. Yi-Xian Qin and held September 30-October 1 in Seattle, Washington, explored current and future research on the mechanism of bone fluid flow as it relates to cellular activities, bone fluid flow imaging, and the translation of these findings
into clinical activities.
The program included the following scientific sessions:
• Fundamental biology and engineering aspects of fluid flow pathways in bone
• Imaging and experimental approaches to determine fluid flow through bone
• Cellular and molecular response to fluid flow stimulation
• Analytical and experimental methods in fluid transportation in bone
• Tissue and cellular fluid flow interaction in bone
• The role of fluid flow in tissue engineering
Speakers
• Lynda Bonewald, Ph.D., Univ. of Missouri at Kansas City. “Correlation of in vitro fluid flow shear stress with in vivo loading”
• Henry Donahue, Ph.D., Penn State University. “Responsiveness of cellular networks to fluid flow”
• Susannah Fritton, Ph.D., City College of New York. “Bone's interstitial Ffluid pathway”
• Robert Guldberg, Ph.D., Georgia Institute of Technology. “Micro-CT imaging of vascularization and mineralization during bone repair”
• Melissa Knothe Tate, Ph.D., Case School of Engineering. “The interplay between mechanics & transport across length scales and its potential role in bone adaptation and remodeling”
• Jenneke Klein-Nulend, Ph.D., ACTA-Vrije Universiteit. “Cell biology of mechano-adaptive bone remodeling: role of bone fluid flow”
• L-Y. Wang, Ph.D., Mount Sinai School of Medicine. “In situ study of solute transport in the bone lacunar-canalicular system”
• Howard Winet, Ph.D., Orthopaedic Hospital/UCLA. “Bone interstitial fluid flow implications for tissue engineering” Program for the ASBMR Fluid Flow in Bone Working Group (Organized by Dr. John Frangos)
• Kenneth McLeod, Ph.D., Binghamton University. “Blood Pressure and Bone Remodeling”
• Yi-Xian Qin, Ph.D., Stony Brook University. “The Role of In Vivo Dynamic Fluid Flow Stimulation in Bone Adaptation”
• Chris Jacobs, Ph.D., Stanford University. “Bone cell mechanotransduction by oscillatory fluid flow”
• Ben Wu, Ph.D., UCLA. “Bone Tissue Engineering”
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Program
Thursday, September 30, 2004
9:30 AM
Welcome Remarks
Session I: Fundamental biology and engineering aspects of fluid flow pathways in bone
9:45 AM
Cytoskeletal strain amplification in the actin filament bundle of osteocyte cell processes
Yuefeng Han1, Stephen C. Cowin1, Mitchell B. Schaffler2, Sheldon Weinbaum1,
1Departments of Mechanical and Biomedical Engineering, CUNY Graduate Center and the City College of New York, New York, NY
2Leni and Peter W. May Department of Orthopedics, Mount Sinai School of Medicine, New York, NY
10:00 AM
Fluid flow shear stress increases assembly and surface expression of connexin
S.Burra1, P. P. Cherian1, E. Sprague2, L.F. Bonewald3, J. X. Jiang1
1Department of Biochemistry1and2Radiology, University of Texas Health Science Center, San Antonio;
3Department of Oral Biology, School of Dentistry, Univ. of Missouri, Kansas City
10:15 AM
Osteocytes sensing dynamic hydrostatic pressure and modulating bone formation response of osteoblasts: a flow independent pathway
E. Takai, M.S. Huang, R.L. Mauck, C.T. Hung, and X.E. Guo
Department of Biomedical Engineering, Columbia University, New York, NY
10:40 AM
Bone's interstitial fluid pathway
Susannah Fritton, City College of New York
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Return to top of page Session II: Imaging and experimental approaches to determine fluid flow through bone
11:05 AM
Micro-CT imaging of vascularization and mineralization during bone repair
Robert Guldberg, Georgia Institute of Technology
11:30 AM
Bio-imaging of intracellular nitric oxide in single cells after mechanical stimulation
A Vatsa1, D Mizuno2, J Klein-Nulend1, CF Schmidt2, FC MacKintosh3, TH Smit4
1Oral Cell Biology, ACTA-Vrije Universiteit,2Biophysics & Complex Systems, Vrije Universiteit,
3Theoretical Physics, Vrije Universiteit, 4Clinical Physics & Engineering, Vrije Universiteit Medical Centre, Amsterdam, The Netherlands
11:45 AM
TBA
12:10 PM
Lunch
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Return to top of page Session III: Cellular and molecular response to fluid flow stimulation
1:30 PM
Correlation of in vitro fluid flow shear stress with in vivo loading
LF Bonewald, J. Feng, J. Gluhak-Heinrich, SE Harris, JX Jiang, S. Kotha, D. Nicolella, M. Schaffler, E. Sprague.
Dept. of Oral Biology, U. of Missouri at Kansas City, Kansas City, MO
1:55 PM
Cell biology of mechano-adaptive bone remodeling: role of bone fluid flow
Jenneke Klein-Nulend, ACTA-Vrije Universiteit
2:20 PM
Responsiveness of cellular networks to fluid flow
Henry Donahue, Penn State University
2:45 PM
Gene expression profiles in an osteocyte cell model: response to fluid flow
Wuchen Yang1, Marie A. Harris2, Dayong Guo1, Lynda F. Bonewald1, and Stephen E. Harris1
1Dept. of Oral Biology, U. of Missouri at Kansas City, Kansas City, MO;
2Dept. of Periodontics, U. of Texas Health ScienceCenter at San Antonio, San Antonio, TX
3:00 PM
Break
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Return to top of page Session IV: Analytical and experimental methods in fluid transportation in bone
3:20 PM
The interplay between mechanics & transport across length scales and its potential role in bone adaptation and remodeling
Melissa Knothe Tate, Case School of Engineering
4:10 PM
Axial and radial intrinsic permeability of cortical bone: indicator of the dynamic loading behavior of bone?
M.A.K. Liebschner1, T.S. Keller2
1Department of Bioengineering, Rice University, Houston, Texas
2Department of Mechanical Engineering, University of Vermont, Burlington, Vermont
4:25 PM
Accumulative effect of oscillatory electromagnetic field (EMF) on top of fluid shear stress on the stress response genes in osteoblast-like cells in vitro
Dajun Zhang1, Reka Letso2, Vikram Sreedharan1, Uday Chippada1, David T. Denhardt2
1Bone Mechanics Lab, Dept. of Mechanical & Aerospace Engineering,
2Dept. of Cell Biology and Neuroscience, Rutgers, State University of New Jersey
4:40 PM
Alteration of long duration repetitive vascular fluid flow as a potential etiology factor of stress fracture
Y. Xia, Y-X. Qin
Department of Biomedical Engineering, State University of New York at Stony Brook, New York
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Return to top of page Session V: Tissue and cellular fluid flow interaction in bone
4:55 PM
In situ study of solute transport in the bone lacunar-canalicular system: a refined two-compartment model
+*Liyun Wang; **Yilin Wang; ***Scott Henderson; *Robert Majeska; *Mitchell Schaffler
+*Leni and Peter W. May Department of Orthopaedics,
***Microscopy Shared Research Facility and Department of Molecular, Cell, and Developmental Biology, Mount Sinai School ofMedicine, New York, NY;
**Department of Biomedical Engineering, CUNY Graduate School and City College of New York, NY
5:15 PM
Simulation of fluorescence recovery after photobleaching in an osteocyte network
David P. Fyhrie1, Roger Zauel1, Harlan Stockman2, Liyun Wang3, Mitchell B. Schaffler3
1Bone and Joint Center, Department of Orthopaedic Surgery, Henry Ford Hospital, **2799 W Grand Blvd., Detroit, MI;
2Division of Geochemistry, Sandia Laboratories, Albuquerque, NM;
3Department of Orthopaedics, Mount Sinai School of Med.,NY, NY
5:35 PM
Disruption of the osteocyte syncytium in osteoporotic bone reduces tissue permeability
R. Steck1, M.L. Knothe Tate2
1Department of Biomedical Engineering, Orthopaedic Research Center,
The Cleveland Clinic Foundation, Cleveland, OH
2Departments of Biomedical Engineering and Mechanical & Aerospace Engineering,
Case Western Reserve University, Cleveland, OH
5:50 PM
Finite element modeling of the response of osteocytic network to oscillatory electromagnetic field (EMF)
Vikram Sreedharan, Dajun Zhang
Bone Mechanics Lab, Dept. of Mechanical & Aerospace Engineering, Rutgers, State University of New Jersey
6:30 PM
Dinner
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Return to top of page Friday, October 1, 2004
7:00 AM
Continental Breakfast
Session VI The role of fluid flow in tissue engineering
7:45 AM
Muscle pump-assisted bone interstitial fluid flow implications for tissue engineering
H. Winet, Orthopaedic Hospital and Depts. Orthopaedic Surgery and Biomedical Engineering, UCLA
8:10 AM
Modeling fluid flow and nutrient transport in cortical bone
Nicolas Hamilton1, Dennis Coombe2, David Tran2and Ronald F. Zernicke1,3
1Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Canada
2Computer Modeling Group, Calgary, Canada
3Faculty of Kinesiology, University of Calgary, Calgary, Canada
8:25 AM
Permeability prediction of tissue engineering scaffolds using mathematical models
MA Wettergreen, MAK Liebschner
Department of Bioengineering, Rice University, Houston, TX, USA
8:40 AM
Response of fluid flow in the lacunocanalicular network to vascular loading
Suzanne Ferreri, Yi-Xian Qin
Department of Biomedical Engineering, State University of New York at Stony Brook
8:55 AM
Role of microstructural variables on the stress generated fluid flow in bone
Subrata Saha and Robert H. Kufahl
Biomedical Materials Engineering Science Program, Alfred University, Alfred, NY
9:10 AM
Bone cell mechanotransduction by oscillatory fluid flow
Christopher R. Jacobs
Department of Mechanical Engineering, Palo Alto Veterans Affairs Medical Center, Stanford University, Palo Alto, CA
9:30 AM
Closing Remarks
Open Discussion
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